导言
类器官技术是用于研究人体器官功能和疾病模型的方法,为了推广这一技术,Bio-protocol 推出了《类器官研究实验方案合集》。该合集汇集了12个国家的18篇类器官研究文章,涵盖大脑、肺、乳腺、肠、肝脏和肾脏等领域的实验研究方案。每篇方案都提供了详细的逐步实验流程,列明所需试剂和设备,并配有图文说明,部分方案还附有实验操作视频。
最后,读者还可以通过在线的“Q&A”模块直接向作者提问。电子合集的下载途径请见文末。
精选集文章索引
● Generation and Maintenance of Homogeneous Human Midbrain Organoids
同种人类中脑类器官的生成与维持
链接:https://doi.org/10.21769/BioProtoc.4049
作者:Henrik Renner、Martha Grabos、Hans R. Schöler、Jan M. Bruder
● Generation of Human Blood Vessel and Vascularized Cerebral Organoids
人类血管和血管化脑类器官的生成
链接:https://doi.org/10.21769/BioProtoc.4870
作者:Xin-Yao Sun、Xiang-Chun Ju、Hong-Fang Zhao、Zhi-Wen You、Run-Run Han、Zhen-Ge Luo
● High Throughput Blood-brain Barrier Organoid Generation and Assessment of Receptor-Mediated Antibody Transcytosis
高通量血脑屏障类器官的产生和受体介导的抗体转胞吞作用的评估
链接:https://doi.org/10.21769/BioProtoc.4399
作者:Elena Kassianidou、Claire Simonneau、Alina Gavrilov、Roberto Villaseñor
● Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells
从多能干细胞生产表型一致的人类大脑类器官
链接:https://doi.org/10.21769/BioProtoc.3985
作者:Adam Sivitilli、Parisa Ghiasi、Liliana Attisano
● Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids
建立双潜能的人肺类器官培养系统及分化产生成熟的肺泡和气道类器官
链接:https://doi.org/10.21769/BioProtoc.4657
作者:Man Chun Chiu、Cun Li、Yifei Yu、Xiaojuan Liu、Jingjing Huang、Zhixin Wan、Kwok Yung Yuen、Jie Zhou
● A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
一个健康的乳腺有机样系统来模拟哺乳和退化样过程
链接:https://doi.org/10.21769/BioProtoc.3996
作者:Elsa Charifou、Jakub Sumbal、Zuzana Koledova、Han Li、Aurélie Chiche
● Antisense Oligonucleotide-mediated Knockdown in Mammary Tumor Organoids
乳腺肿瘤类器官中反义寡核苷酸介导的基因敲低技术
链接:https://doi.org/10.21769/BioProtoc.2511
作者:Sarah D. Diermeier、David L. Spector
● Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability
小鼠结肠和小肠类器官的制备和培养,包括基因表达和类器官活力的分析
链接:https://doi.org/10.21769/BioProtoc.4298
作者:Luisa Klemke、Joshua P. Blume、Tiago De Oliveira、Ramona Schulz-Heddergott
● Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids
用于人结直肠上皮类器官和类肿瘤的低粘度基质悬浮培养
链接:https://doi.org/10.21769/BioProtoc.4394
作者:Tao Tan、Yumiko Hirokawa、Jordan Clarke、Anuratha Sakthianandeswaren、Oliver M. Sieber
● In vitro Self-organized Mouse Small Intestinal Epithelial Monolayer Protocol
小鼠小肠上皮单层膜体外自组装实验方案
链接:https://doi.org/10.21769/BioProtoc.3514
作者:Gizem Altay、Eduard Batlle、Vanesa Fernández-Majada、Elena Martinez
● Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids
基于Fluidigm的源于患者小肠类器官单细胞基因表达文库的制备
链接:https://doi.org/10.21769/BioProtoc.3775
作者:Kohei Suzuki、Ryuichi Okamoto
● From 3D to 2D: Harmonization of Protocols for Two-dimensional Cultures on Cell Culture Inserts of Intestinal Organoids from Various Species
从三维到二维:不同物种肠道类器官细胞培养插件的二维培养方法的协调
链接:https://doi.org/10.21769/BioProtoc.4295
作者:David Warschkau、Estefanía Delgado-Betancourt、David Holthaus、Antonia Müller、Gudrun Kliem、Susanne M. Krug、Joerg-Dieter Schulzke、Frank Seeber、Toni Aebischer、Christian Klotz
● Generation, Maintenance and HBV Infection of Human Liver Organoids
人肝类器官的产生、维持和HBV感染
链接:https://doi.org/10.21769/BioProtoc.4358
作者:Shahla Romal、Tanvir Hossain、Tokameh Mahmoudi
● Production of Recombinant Hepatitis B virus (HBV) and Detection of HBV in Infected Human Liver Organoids
重组乙型肝炎病毒 (HBV) 的产生和受感染的人类肝脏类器官中 HBV 的检测
链接:https://doi.org/10.21769/BioProtoc.4392
作者:Tanvir Hossain、Shahla Romal、Tokameh Mahmoudi
● In situ Hybridization of miRNAs in Human Embryonic Kidney and Human Pluripotent Stem